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KMID : 0364819870250040274
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Korean Journal of Microbiology
1987 Volume.25 No. 4 p.274 ~ p.281
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Improvement of the regeneration and protoplasts fusion of Candida pseudotropicalis by bovine serum albumin, myoinositol and ergosterol
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Abstract
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1
The a feet of bovine serum abunda, agobodtot and er=osteroi on protopWt forma. don, repareradan sad fadoa from aQOtrophie amtane of Candida p eudotroplca& were esaadaed. Frequency of protoplrt foramtion ranged from 411 to 90% depending on aa:otrophk types. When aq
ataortol903 mg/m) and ataderei (0.1 mg/ml) was sapplemamed to the medium, of an grow*, and bovine scram albuada (4 mg/ no wa added to protopbsdog buffer, 50-111% of ab were con
verted to protoplrb. Suck a treadaent of three addUhes improved 2.2.3.4 fold of resemeration rate of protopbse. The tudoa frequencies between oompiemenNT aa:otropto ranged from 7.0 x 104 to 1.5 x 10´3 in the optbml conditions. These ~aims showed 1.9-23 fold increase when compared with tod" frqueecies obtained without the treatment of addidv s These rauib suggested that these compounds MY improve protopbst reparation and fudou between complemeahry araotropb used L tW study.
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